The Journal of Scanning Microscopies


Search the SCANNING website

SPI Supplies: Your Quality Source Note to authors:
All new manuscripts must be submitted online at:

SCANNING VOL. 27, 136–140 (2005)
Received: August 16, 2004
Accepted with revision: November 29, 2004
© FAMS, Inc.

Scanning Force Microscopy Studies of the Colonization and Growth of A. ferrooxidans on the Surface of Pyrite Minerals

D. L. Pace, R. E. Mielke,* G. Southam,† T. L. Porter

Department of Physics and *Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, USA; †Department of Earth Sciences, University of Western Ontario, London, Ontario, Canada

Full-text (for Scanning subscribers)

Summary: We have used the technique of scanning force microscopy (SFM) for studying the interaction of the bacteria A. ferrooxidans with the surface of the mineral pyrite. These bacteria are important to study with regard to acidification of streams and the environmental impact of such acidification. A. ferrooxidans cells readily colonize the pyrite surface, forming a tight mineral seal between the cell and the pyrite substrate. These bacteria subsequently may grow under pH neutral conditions, biooxidizing the underlying pyrite; this process creates etch pits in the pyrite. On average, these etch pits are 1.2 microns in lateral dimension and approximately 220 nm deep.

Key words: scanning force microscopy, pyrite, ferrooxidans

PACs: 61.16.-d, 87.15.-v

Address for reprints:
T. L. Porter
Northern Arizona University
Department of Physics
Box 6010
Flagstaff, AZ 86011, USA